Biochar (BC), nitrification inhibitors (methyl 3-(4-hydroxyphenyl) propionate, MHPP), and urease inhibitors (n-butyl phosphorothioate triamine, NBPT) have emerged as effective soil greenhouse gas (GHG) mitigation strategies in agroecosystems. However, the combined use of BC and inhibitors in karst areas has no available data. Therefore, the combined effects of BC, MHPP, and NBPT on GHG emissions, global warming potential (GWP) and nitrogen use efficiency (NUE) in roasted tobacco cropping systems were studied to improve the understanding in climate mitigation. CO2, CH4, and N2O emissions from soils were measured using static chamber-gas chromatography. Results showed that the combined use of BC and inhibitors significantly increased soil total nitrogen, available potassium, electric conductivity, pH, and soil organic matter compared to the control. The combined use of BC and MHPP or NBPT significantly increased cumulative soil CO2 emissions by 33.95% and 34.25%, respectively. The exponential-exponential function of soil CO2 fluxes with soil moisture and temperature demonstrated good fit (R-2: 0.506-0.836). The combination of BC and NBPT increased the cumulative soil CH4 emissions by 14.28% but not significantly compared to the fertiliser treatment. However, the combination of BC and MHPP resulted in a significant reduction in cumulative soil CH4 emissions by 80.26%. In addition, the combined use of BC and MHPP or NBPT significantly reduced the cumulative soil N2O emissions by 26.55% and 40.67%, respectively. The inhibition effect of NBPT was better than MHPP. Overall, the combined use of BC and inhibitors significantly reduced the yield-scaled GWP, markedly increased crop yield and NUE, and mitigated climate change in the southwest karst region.

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