To characterize the integrated magmatic processes for magma plumbing systems from mantle sources to magma chambers, we present Mg-Sr-Nd isotopic data for a representative mafic-ultramafic intrusion in Xiarihamu, northern Tibetan Plateau, China. The intrusion hosts the largest magmatic Ni-Cu sulfide deposit in an orogenic setting in the world and is composed predominantly of harzburgite, orthopyroxenite, websterite, and gabbronorite. The cumulate harzburgites have relatively low delta Mg-26 of orthopyroxene (-0.49 to -0.34 parts per thousand) and moderately high (Sr-87/Sr-86)(i) and positive epsilon(Nd)(t) values (0.7067-0.7080 and +0.4 to +1.8, respectively). Most of the orthopyroxenites, websterites, and gabbronorites have slightly higher delta Mg-26 of orthopyroxene (-0.34 to -0.21 parts per thousand) and higher (Sr-87/Sr-86)(i), and lower epsilon(Nd)(t) values (0.7087-0.7118 and -4.0 to -1.4, respectively) than the harzburgites. On the other hand, marginal facies (i.e. a few centimeters to meters from the contact between the intrusion and country rocks of schist, gneiss, and marble) including websterite and gabbronorite are marked by low delta Mg-26 of orthopyroxene (-0.44 to -0.33 parts per thousand) and extremely high (Sr-87/Sr-86) ; and negative epsilon(Nd)(t) values (0.7127-0.7172 and -5.4 to -4.8, respectively). The relatively low delta Mg-26, moderately high (Sr-87/Sr-86)(i), and positive epsilon(Nd)(t) of the harzburgites are best interpreted by inheritance from the mantle source, which was previously metasomatized by subducted Mg-rich carbonates. Given the fact that the solidus of carbonated mantle is significantly lower than that of volatile-free mantle, mantle metasomatism by carbonates may be critical to generate voluminous mafic magmas and to form the giant Xiarihamu Ni-Cu deposit and other magmatic sulfide deposits in convergent settings worldwide. The relatively high S 26 Mg, high (Sr-87/Sr-86)(i), and low epsilon(Nd)(t) of most orthopyroxenites, websterites, and gabbronorites were produced by extensive contamination of isotopically heavy crustal rocks in a deep-seated magma chamber. The slightly low delta Mg-26, extremely high (Sr-87/Sr-86) ; and negative epsilon(Nd)(t) of the marginal facies, compared with other websterites and gabbronorites, were governed by assimilation of local low-delta Mg-26 country rocks (e.g. gneiss and marble) at the Xiarihamu magma chamber during emplacement. These observations indicate that mafic-ultramafic cumulates away from marginal facies can preserve their primitive Mg-Sr-Nd isotope signatures inherited from deep magma chambers, even from mantle sources, with negligible crustal contamination during magma ascent and emplacement. These findings for the first time, to our knowledge, provide observational evidence to characterize the integrated magmatic processes of a single magma plumbing system from deep mantle source to magma chambers, which sheds light on the petrogenesis and mineralization of mafic-ultramafic intrusions.