The Yongxin gold deposit is one of the prominent gold deposits in the Duobaoshan gold-copper metallogenic belt within the eastern domain of the Central Asian Orogenic Belt. The deposit has recently been discovered therefore, its genesis and economic significance remain unknown. Here, we present the results of pyrite thermoelectricity, fluid inclusion and H-O-Rb-Sr isotopic studies of different minerals with a view to decipher ore genesis and ore prospecting in the Yongxin gold deposit. The Rb-Sr isotopic composition of pyrite, quartz and calcite indicate that the deposit was formed at 114.6 +/- 1.2 Ma. Fluid inclusions and H-O isotopic studies show that the Yongxin gold deposit was formed at shallow depth, with relatively low ore-forming temperatures (120-355 degrees C), low-medium salinity (0 wt% to 10 wt%), and low-moderate density (0.6-1.0 g/cm(3)). The ore forming fluids were mainly magmatic which mixed with meteoric water triggering the precipitation of ore forming materials. We trace the evolution of the ore fluids from deeper levels in the northwest part of the deposit to shallower levels in the southeast. The thermoelectricity parameter values (Xnp) of pyrite range from -188 to -85 and the values of erosional or exhumation level (gamma) range from 97% to 71% with an average of 79.4%, suggesting that the existing ore-body is the lower part of the deposit (79.4% of the ore-body has already been eroded). Thermoelectricity of pyrite shows a vertical distribution in the axial direction without significant change in the Xnp values, indicating that the ore body extends further. Combined with the pyrite thermoelectric coefficients in different altitudes, we predict gold potential for the ores at further depth depending on the continued presence of the N-P type pyrite. Our mineralogical mappings also suggest a prospecting potential in the two zones at depth beneath the northwestern part of the deposit.