To investigate the sources and spatial-temporal distribution of dissolved heavy metals in river water, and to evaluate the water quality, a total of 162 water samples were collected from 81 key sampling points in high and low flow seasons separately in the Zhujiang River, Southwest China. Ten dissolved heavy metals (V, Cr, Mn, Co, Ni, Cu, Mo, Cd, Ba, and Pb) in the Zhujiang River water exhibit little variation at temporal scale, but vary with a significant spatial heterogeneity. Furthermore, different metals present different variation trends along the main channel of the Zhujiang River. Our results suggest that Ba (14.72 mu g L-1 in low flow season and 12.50 mu g L-1 in high flow season) and Cr (6.85 mu g L-1 in low flow season and 7.52 mu g L-1 in high flow season) are consistently the most abundant metals in the two sampling periods. According to the water quality index (WQI values ranged from 1.3 to 43.9) and health risk assessment, metals investigated in Zhujiang River are below the hazard level (all hazard index (HI) < 1). Application of statistical approaches, including correlation matrix and principal component analysis (PCA), identify three principal components that account for 61.74% of the total variance, the results conclude that the anthropogenic heavy metals (V, Cr, Ni, and Cu) are greatly impacted by the dilution effect, and the heavy metals in Zhujiang River are mainly presented a natural sources signature from the perspective of entire basin. Moreover, our results reveal that the estimated export budget of several heavy metals including V (735.6 t year(-1)), Cr (1,561.1 t year(-1)), Ni (498.2 t year(-1)), and Mo (118.9 t year(-1)) to the ocean are higher than the world average.