In order to construct the indicators of the balance between supply and demand of the cultivated land ecological carrying capacity, basing on the relation of the cultivated land ecological carrying capacity supply and demand, applying the model of Cultivated Land Ecological Footprints and the method of CIS and considering the factors of cultivated land production, taking the statistical data of 2015 as an example, and then made a systematic evaluation of the balance between supply and demand of the cultivated land ecological carrying capacity in Guizhou Province. The results show that (1) the spatial distribution of supply and demand of cultivated land ecological carrying capacity in Guizhou is unbalanced, and the northern and eastern parts are the overloading area, the middle, the south and the west parts are the balance area. (2) From the perspective of cultivated land structure, the crops with ecological carrying capacity surplus were rice, vegetables and peanuts, among which rice was the highest and the ecological balance index was 0.7354. The crops with ecological carrying capacity overload were potato, wheat, maize, rapeseeds, soybeans and cured tobacco, of which the index of potato up to 7.11, other types of indices are less than 1.5. The research can provide the ecological security early warning, the overall plan of land use and sustainable development of the area cultivated land with scientific evidence and decision support.