Effect of digestion methods on fluorescence intensity of fluorescent polystyrene (PS) beads was poorly understood, which may affect the accuracy of toxicity test of the fluorescent PS beads exposed to marine organisms. Therefore, six digestion approaches were compared on fluorescence intensities and properties of three commercial fluorescent PS beads. Among all the protocols, the digestion using KOH (10% w/v, 60 degrees C) (KOH-digestion) had no effect on the fluorescence intensity, morphology and composition of the three fluorescent PS beads. Moreover, the extraction efficiency >= 95.3 0.2% of fluorescent PS beads in Daphnia magna and zebrafish, confirming its feasibility in fluorescent PS beads quantitative analysis. However, the fluorescence intensities of fluorescent PS beads digested by other five protocols were significantly decreased, as well as the change of morphology and composition on fluorescent PS beads. Overall, the KOH-digestion is an optimal protocol for extracting fluorescent PS beads in biological samples. Publication name | MARINE POLLUTION BULLETIN, 131 515-524; 10.1016/j.marpolbul.2018.04.056 A JUN 2018 | Author(s) | Li, Fengmin; Li, Fuyun; Hou, Xiaodong; Luo, Xianxiang; Tu, Haifeng; Zou, Yadan; Sun, Cuizhu; Shi, Mei; Zheng, Hao | Corresponding author(s) | ZHENG Hao zhenghao2013@ouc.edu.cn Ocean Univ China, Inst Coastal Environm Pollut Control, Qingdao 266100, Peoples R China Ocean Univ China, Minist Educ, Key Lab Marine Environm & Ecol, Qingdao 266100, Peoples R China Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Peoples R China | Author(s) from IGCAS | HOU Xiaodong | View here for the details
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