The Jurassic-Early Cretaceous Yilashan mafic-ultramafic complex is located in the middle part of the Bangong-Nujiang suture zone, central Tibet. It features a mantle sequence composed of peridotites and a crustal sequence composed of cumulate peridotites and gabbros that are intruded by diabases with some basalts. This article presents new whole-rock geochemical and geochronological data for peridotites, gabbros, diabases and basalts to revisit the petrogenesis and tectonic setting of the Yilashan mafic-ultramafic complex. Zircon laser ablation inductively coupled plasma mass spectrometer (LA-ICP-MS) U-Pb ages of three diabase samples are 169.6 +/- 3.3Ma, 132.5 +/- 2.5Ma, and 133.6 +/- 4.9Ma, respectively. These ages together with previous studies indicate that the Yilashan mafic-ultramafic complex probably formed during the Jurassic-Early Cretaceous. The peridotites exhibit nearly U-shaped REE patterns and are distinct from abyssal peridotites. The diabase and basalt samples show arc features with selective enrichment in light rare earth elements (LREE) and large ion lithophile elements (LILEs; e.g. Rb, U, and Sr) and depletion in high field strength elements (HFSEs; e.g. Nb, Ta, and Ti). The gabbro samples display cumulate features with selective enrichment in LILEs (e.g. Rb, Ba, and Sr) but depletion in LREEs and HFSEs (e.g. Nb, Zr, and Ti). Combing the positive epsilon(Nd)(t) values (+6.1 to +10.0) and negative zircon epsilon(Hf)(t) values (-16.5 to -11.7 and -13.6 to -0.4) with older Hf model ages for the mafic rocks, these signatures suggest that the Yilashan mafic and ultramafic rocks likely originated from an ancient lithospheric mantle source with the addition of asthenospheric mantle materials and subducted fluids coupled with limited crustal contamination in a continental arc setting as a result of the southward subduction of the Bangong-Nujiang Tethys Ocean beneath the Lhasa terrane during the Jurassic-Early Cretaceous.