LI Wei 1,2, ZENG Xian-dong1, LI Mao-teng1,2, Zhou Peng-peng1,2, YU Long-jiang1,2
1.Institute of Resource Biology and Biotechnology,Department of Biotechnology,College of Life Science and Technology,Huazhong University of Science & Technology,Wuhan 430074,China; 2.Key Laboratory of Molecular Biophysics,Ministry of Education,Wuhan 430074,China
Abstract: Chlamynonas reinhardtii was selected as the target microalgae and was inoculated onto simulative soillimestone columns.T his study systematically measured dynamics of electric conductivity,Ca2+ concentration and carbonic anhydrase activity in leachates under different leaching conditions. The results showed that,when the columns were leached with redistilled water,the total amount of leached Ca2+ at the time of 24dwas 11.9%of that leached with culture liquid of microalgae, and that the average activity of carbonic anhydrase in the leachates was correlated well with the total amount of leached Ca2+.These results implied that microalgae and its carbonic anhydrase have significant driving effect on calcium migration in the soil-limestone karst system. The results of this paper provide scientific basis for studying the role of algae carbonic anhydrase in karst development. It is expected that a deepen investigation following this study will help in clarifying the role of algae and the carbonic anhydrase in carbon sequestration of karst areas.
Key words: soil-limestone system;leaching;Ca2+; carbonic anhydrase;element migration
*Corresponding Author, E-Mail: yulongjiang@mail.hust.edu.cn
Bulletin of Mineralogy, Petrology and Geochemistry Vol. 30, No.3, 2011, page 261-264
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