Abstract: This study investigated the pollution state of antimony in the waters of an antimony mine located in Banpo, Guizhou Province, and traced the pollutant s using sulfur isotope. The main goals of this s tudy are to clear the geochemical progresses controlling the fate and the transfer of antimony and to better underst and the current pollution state of the rivers in this antimony mine area. The result s showed that the waters are suffering from serious pollution with Sb and SO42- concentrations being up t o 1377 µg/ L and 1926 mg/ L, respectively. The downstream water of the Chahe River, 10 km away from the mine area, still contains high concentrations of Sb and SO42-.  In Chahe River, δ34 S-Sb, δ34 S-SO42- and Sb-SO42- are remarkably positively correlated, the correlation coefficients are r= 0. 68( p< 0. 05) , r= 0. 89( p< 0. 01) and r= 0. 72( p < 0. 05) , respect ively. These positive correlations suggest that δ34S in the dissolved sulfat e and the concent rat ion of SO42- can well reflect the water pollution state and the diffusion distance of antimony in the Chahe River.  According to the calculation of isotope mass balance, the sulfur in the downstream of the mine area are mainly form antimony deposit , which implies that the water quality in the mine area is significantly affected by mining activities.