CHEN Huiqing1,2, Xiaochen1, YU Xuefeng2, MA Yu3, KE Yang3, ZHU Haiyun3, XUE Jing1
（1. College of Life Science, Shaanxi Normal University, Xi’an, 710119, China;
2. State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an, 710061, China;
3. Shaanxi Microbiology Institute, Xi’an, 710043, China;）
Abstract: As the principle part of soil biodiversity, the study of soil microbial diversity is mainly focusing on the categories, abundances, distributions and diversities of microbial community reflecting the diversity of soil microbial communities. At the end of the 19th Century, the conventional microbial cultivation methods were applied in the analysis of soil microbial diversity. Then, the biochemical methods, represented by PLFA (phospholipid fatty acid) analysis and BIOLOG microanalysis, were used for the evaluation of soil microbial community diversity. With the rapid development of molecular biology technologies in subsequent decades, related research methods such as denatured gradient gel electrophoresis (DGGE), terminal-restriction fragment length polymorphism (T-RFLP), cloning library and high-throughput sequencing were widely employed in research of soil microbial diversity. This paper reviews the literature on the soil microbial diversity research technology, including the principles and developments. The advantages and disadvantages of different technologies were compared and their application fields were also summarized. The research fields of soil biodiversity warranting further attentions were also discussed.
Key words: microbial diversity; soil ecosystem; microbial community; review
EARTH AND ENVIRONMENT Vol.46, No.2, Tot No.322, 2018, Page 204-209
