LIU Xing 1,2,3, WANG Shijie1,3,*, LIU Xiuming1,3, HUANG Tianzhi1,2,3, LI Yong1,2,3
(1. State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry,Chinese Academy of Sciences, Guiyang 550180, China;
2. University of Chinese Academy of Sciences, Beijing 100049,China;
3. Puding Karst Ecosystem Research Station, Chinese Academy of Sciences,Puding 562100,China)
Abstract:By using high-throughput pyrosequencing technique, this paper investigated the soil microbial abundancesandcommunitystructure in 5 successionstages (sparse-shrubtussock, thornyshrub, shrub, shrub-treetransitionalforest, and secondaryforest) in the Puding Karst area,central of GuizhouProvince, China, and analyzed the main environmental factors affecting these changes. Results showed that in all the soil samples, procaryote microbial was dominated with Proteobacteria(43.35%), Acidobacteria(12.97%), Verrucomicrobia(7.53%),Actinobacteria(7.12%), Firmicutes(6.19%), Bacteroidetes(5.35%), and Planctomycetes(5.05%), while unclassified was 20.65%. The few low abundance of Euryarchaeota and Crenarchaeotawere detected in the Karst area soil. in Zanthoxylumplanispinum rhizosphere soil, the abundance of Proteobacteria, Firmicutes and Planctomycetegradually increased with the vegetation succession. However, in the non-rhizosphere soil, the abundance of Acidobacteria and Verrucomicrobiadecreased from 14.74% to 12.92% and 11.29% to 6.11%, respectively. It was figured out that TOC, TN, soil water content, EC, available phosphorus content, pH and exchangeable calcium content were key factors affecting the microbial community in the karst soil, and TOC and TN were significantly different.
Key words:soil bacterial; high-throughput sequencing; vegetation succession; rhizosphere; karst
EARTH AND ENVIRONMENT Vol. 43, No.5 Tot No.307, 2015, Page 490-497
