WANG Kun1，FANG Min2，WU Chenxi2，NING Zengping3，WAN Yun1

(1. China coal Pingshuo Corporation, Shuonan Development and Construction Headquarters, Shuozhou 036006, China;
2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
3. Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China)

Abstract: Method for the analysis of steroid hormones in water and soil using liquid chromatography mass spectrometry (LC-MS) was established. Water samples were concentrated using solid phase extraction (SPE) while soil samples were first extracted using accelerated solvent extraction (ASE) and then purified and concentrated using SPE. Results show that the method detection limits were 0.01－0.6 ng/L and 0.01－0.19 ng/g, the recoveries were 77%－97% and 62%－119%，and the RSD are lower than 6.04% and 15.5%, respectively for the analysis of steroid hormones in water and soil. This method is accurate and precise and suitable for determining the residues of steroid hormones in water and soil.

Key words: steroid hormone; LC-MS/MS; derivatization; solid phase extraction; accelerated solvent extraction

EARTH AND ENVIRONMENT Vol. 43, No.3, Tot No.305, 2015, Page 369-374