WANG Rui^{1, 2}, WU Yanyou^{1, 3*}, XING Deke³, HANG Hongtao¹, LIU Ying¹, ZHANG Kaiyan¹, YAN Kai¹

 (1.State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China;
2. Food and Pharmaceutical Engineering Institute, Guiyang College, Guiyang 550005, China;
3. Key Laboratory of Modern Agricultural Equipment and Technology，Ministry of Education & Jiangsu Province, Institute of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China

Abstract: To screen available energy plant for the Karst marginal land, plant physiological and biochemical and geochemical technique were introduced into biomass field. Orychophragmus violaceus L., Brassica juncea L., and Euphorbia lathyris L. were stressed under HCO₃^-(10 mmol/L）, a simulative Karst adversity. During the whole experiment, index of three kind of plant leaves were determined regularly, including proline, malondialdehyde, chlorophyll, chlorophyll fluorescence, diurnal variation of photosynthesis, carbonic anhydrase activity, stable carbon isotope composition of leaves, and biomass production. In contrast, Orychophragmus violaceus L. and Brassica juncea L. are more sensitive than Euphorbia lathyris L. under HCO₃^- stress, but Euphorbia lathyris L. is easily affected by low temperature. Euphorbia lathyris L. shows stronger resistance and photosynthetic performance at proper temperature. Results show that Orychophragmus violaceus L. shows the highest inorganic carbon-usage capacity because of high Karst adaptability and carbonic anhydrase activity. Considering that the high biomass production, Orychophragmus violaceus L. is highly recommended as a biomass feedstock in Karst marginal land.

Key words: Karst environment; bicarbonate; stress; biomass plant

EARTH AND ENVIRONMENT Vol. 43, No.1, Tot No.303, 2015, Page 21-30