CHENG Su-chun, CHEN Tian-hu, WANG Jin, YUE Zheng-bo, ZHOU Yue-fei, LUO Xiao-xiao

(Laboratory for Nanomineralogy and Environmental Material, School of Resources and Environmental Engineering,

Hefei University of Technology, Hefei 230009, China)

Abstract: Xiangshan Landfill was constructed above the iron sulfides tailings, mixed with porphyrite iron ore tailings and acidic drainage in Ma’anshan City, China. The landfill is rich in organic waste, sulfates and iron oxide waste. Furthermore, microbial electron acceptor and donor are co-existed there for a long time. Study on microorganism-mineral interaction of this special system is of great importance. Hence, the microorganisms in the mixed area of landfill and tailings in Xiangshan were cultivated. A pure bacteria strain was isolated after enrichment culturing and plate streaking. Optical microscopy, scanning electron microscope, 16SrDNA sequencing and reduction of sulfate ability test were conducted to identify the bacterial strain as Clostridium Sulfate Reducing Bacteria (short for C. SRB). The results of the physiological-biochemical characteristic investigate of this bacteria show that it grows with glucose and lactose as carbon source, methyl red test result shows negative, while both indole test and catalase test results show positive. Besides, the growth and metabolism of the bacteria strain and the external environment factors including temperature, pH, salinity, and etc were studied. It was found that the highest ratio rate for the sulfate consumption is 0.73 mmol·g^-1·h^-1 when C. SRB was cultivated for 36 h, and the optimum growth conditions are temperature of 35 ℃, pH of 7 and salinity of 0.5 g/L.

Key words: landfill; tailing; sulfate reducing bacteria; interaction of microbe and minerals; physiological and biochemical characteristics

ACTA MINERALOGICA SINICA Vol. 34, No. 2, 2014, Page 175-180